ScienceDirect.com - Developmental Biology - At most three ES cells contribute to the somatic lineages of chimeric mice and of mice produced by ES-tetraploid complementation: "Classical experiments in the 1970s were undertaken to determine how many cells in the mouse embryo are set aside at the time of allocation to form a given lineage. The predominant method to determine the number of founder cells was the generation of chimeric mice by aggregation of preimplantation mouse embryos carrying different genetic markers. Based upon binomial or trinomial calculations, the contribution of the parental genotypes to the chimeras leads to the conclusion that the epiblast lineage is formed by a minimum of three founder cells"
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